Dr. Gabriel Knudsen is currently a Senior Research Chemist in the Analytical Chemistry and Pharmaceutics Group (ACP) within Discovery Sciences at RTI International, and he is a DABT. At RTI, he is an investigator responsible for studying the absorption, distribution, metabolism, and excretion (ADME) of xenobiotics in mammals for industrial clients and for the National Toxicology Program (NTP) and other branches of the National Institutes of Health (NIH). Dr. Knudsen has experience in the preparation and substrate testing using bacterially expressed CYP enzymes, preparation and testing of hepatic microsomal protein and primary cell isolations (notably, hepatocytes, cardiomyocytes, and brain capillaries). He has investigated dermal uptake and metabolism of known and suspected endocrine disruptors using in vitro and in vivo models. He has extensive experience working with ex vivo tissue preparations and organ baths for assessment of biological responses from a variety of models, from human skin to rodents and arthropods. His research has been published in more than 30 peer-reviewed journals focusing on chemical metabolism, pharmacokinetic modeling, and biochemical toxicology of probable endocrine disruptors.
Dr. Knudsen has extensive experience leading and managing teams and projects and experience with applying his expert knowledge of and expertise with bioanalytical, toxicological, pharmacokinetic (PK), and physiologically based pharmacokinetics (PBPK) modeling. He has experience with performing rodent in vivo PK, including designing PK studies, writing protocols, developing formulations, conducting bioanalysis and interpreting data for publications, presentations, and/or submissions to relevant government agencies and contract clients for investigation of small molecules and purified proteins and peptides. He is also an active mentor and mentee, mentoring and training technicians and undergraduate and graduate students on how to keep laboratory records and reporting, how to use in vitro methods, how to handle hazardous materials, and how to use analytical chemistry techniques. From 2014 to 2019, he selected, mentored, and trained NIH post-baccalaureate Fellows on how to apply in vitro and in vivo experimental techniques during toxicological and ADME testing.